Lung gamma delta T cells in tumor-free and mammary tumor-bearing mice

Gamma delta T cells were sorted from the lungs of 4 female FVB/n mice (labeled wild-type [WT] 1-4) and 4 female mammary tumour-bearing K14-Cre;Brca1F/F;Trp53F/F (KB1P) mice (labeled Tumour 1-4).The sorted cells were loaded onto the Chromium Single Cell 30 Chip Kit v2 (10xGenomics) to generate libraries for scRNAseq, following the manufacturer's instructions. The sequencing-ready library was cleaned up with SPRIselect beads (Beckman Coulter, High Wycombe, UK). Quality control of the library was performed prior to sequencing (Qubit, Bioanalyzer, qPCR). Illumina sequencing was performed using NovaSeq S1 by Edinburgh Genomics (University of Edinburgh).