Observe the role of AQP1 gene overexpression in the RENCA cell line

Aquaporin-1 (AQP1) has been implicated in tumor progression, including cell migration, invasion, and angiogenesis in various cancers, but its specific role in renal clear cell carcinoma remains incompletely understood. To investigate the transcriptomic changes associated with AQP1 overexpression in renal cancer, we generated stable AQP1-overexpressing RENCA cells (a murine renal cell carcinoma cell line) and corresponding negative control cells using lentiviral transduction. Two weeks post-transduction, total RNA was extracted from three biological replicates per group and subjected to paired-end high-throughput RNA sequencing (RNA-seq).This dataset provides transcriptome profiles comparing AQP1-overexpressing RENCA cells to negative control cells, aiming to identify differentially expressed genes, pathways, and potential mechanisms by which AQP1 influences renal cancer biology. The data may facilitate further studies on AQP1 as a therapeutic target or biomarker in renal clear cell carcinoma.