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mouse C2C12 cell transcriptome sequencing

NIAID Data Ecosystem2026-03-11 收录

肌细胞分化

NMD无义介导的mRNA衰变

数据链接：

https://www.ncbi.nlm.nih.gov/bioproject/PRJNA548061 数据链接链接失效反馈

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资源简介：

we performed the high-throughput RNA sequencing to investigate the effect of NMD on myoblast differentiation by knockdown the key NMD factor, up-frameshift 1 (UPF1)

应用场景：

创建时间：

2019-06-10

基因表达分析

肌细胞分化

Real-time qPCR primers for selected genes. (XLS 38Â kb)

DataCite Commons2020-08-30 更新60

Protein synthesis during myoblast differentiation

蛋白质代谢

肌细胞分化

Mole (MSR) and fractional (FSR) synthesis rates of proteins during C2C12 myoblast differentiation were investigated. Myoblast cultures supplemented with D2O during 0-24 h or 72-96 h of differentiation

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Fig4a MYOG and MHC co-IF C2C12

肌细胞分化

分子标记

In vitro C2C12 differentiation. Analysis by Myogenin (MYOG, green) and myosin heavy chain (MHC, all isoforms, red) co-staining. Cells were counterstained with DAPI (blue).

Figshare2025-12-21 更新30

Global SLAM-Seq for accurate mRNA decay determination and identification of NMD targets. Global SLAM-Seq for accurate mRNA decay determination and identification of NMD targets

RNA降解动力学

NMD无义介导的mRNA衰变

Gene expression analysis requires accurate measurements of global RNA degradation rates, earlier problematic with methods disruptive to cell physiology. Recently, metabolic RNA labeling emerged as an

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Transcriptomic Analysis of Differentiating Primary Myoblasts treated or not with TGFB1 recombinant protein or ITD-1 compound.

肌细胞分化

TGFβ信号通路转录调控

Transforming Growth Factor beta (TGFβ) signaling cascade plays a fundamental role during muscle development and regeneration. Previous reports proposed several roles for the TGFβ superfamily members a

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mouse C2C12 cell transcriptome sequencing

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