The GWAS summaries of 10 muscle metabolites in cattle with a genome-wide significance threshold of P < 5 × 10 ^-8

The genotyped with Illumina Bovine 770K Bead chip were imputed to sequence level using Beagle 5.4 based on a multiple-breed reference population consisted of 1,847 individuals from run 8 of the 1000 Bull Genomes Project. A total of 8.2 million variants (MAF > 5% and missing rate < 10%) for 116 individuals were used to perform the genome-wide association analysis. Before running the GWAS, the metabolite abandance with log2 transformation were adjusted for age (month), batch and the first two principal components produced by PLINK 1.9. The associations among variants and metabolites were tested using mixed linear models in GCTA software.