Physical characteristics of DIA1R proteins and similarity to orthologues from key species.

aSee Table S5 for accession numbers of full length DIA1R proteins.bIsoelectric point calculated using the assumption that all residues have pKa values equivalent to that of isolated residues, so may not accurately represent the value for the folded protein.cIsotopically averaged molecular weight prediction in kiloDaltons.dThe BLASTP E-value (Expect value) measures the statistical significance threshold for protein sequence matches. The smaller the number, the better the match. Computer shorthand nomenclature is used to present E-values when values are small. For example, 5e-01 = 0.5 and 5e-04 = 0.0005. Values lower than 1e-250 are treated as zero. A dash is used when alignments have 100% identity.eProteins were compared to DIA1 (or DIA1a when paralogues were present) and DIA1R from Homo sapiens, Gallus gallus or Danio rerio by BLASTP. The proteins used for comparison were chosen as representatives from the Class Mammalia, Class Aves, and Class Neopterygii within the subphylum Vertebrata.fDIA1R orthologues are only found in the subphylum Vertebrata and not in other subphyla or phyla. For example, DIA1R orthologues are not found in the phylum Nematoda, Platyhelminthes, Cnidaria, Echinodermata, or Arthropoda.