Putative novel wheat miRNAs discovered by Wei et al. [17] identified in chromosome 1AL survey sequences.

1This pre-miRNA had an identical wheat EST match.2pre-miRNA sequence matched a transposable element, but only one copy was found in 1AL.3miR2023 and miR5050 (see Tables 1 & 2) derive from opposite arms of the same miRNA:miRNA* duplex.4miR2032 is identical to miR5200 (see Table 1).5Mismatches to the conserved miRNA sequence are underlined and in bold.6MFE = Minimum Folding free Energy of predicted hairpin secondary structure.7MFEI = Minimum Folding Energy Index, calculated as described by Yin et al. [40].