Comparison of the neutralizing antibody responses (PRNT50) elicited by the VLP vaccine and controls.

A summary of geometric mean titers of PRNT50 GMT for comparison between the two ZIKV strains, MR-766 and FSS 13025 for both vaccine and virus control groups. Neutralizing activity elicited by the VLP vaccines against the two viruses were comparable, except the VLP for 1μg alone, which had greater activity against MR766 than that of FSS (a p = 0.0033). The In-ZIK virus control 4 μg plus adjuvant also showed a difference between the two viruses (b p = 0.026). The statistical analysis was performed using t-test at confidence intervals of 95% with GraphPad Prism 4 software. Human serum isolated from a patient who recovered from a Zika infection was used to validate PRNT technique. However, no statistical analysis can be performed to compare the neutralization with two different Zika viruses due to the low sample size.