A customized whole-genome DNA microarray of Clostridioides difficile 630∆erm was used (8x15K format, Agilent). Quadruplicate samples were analysed for the DNA microarray. Using the ULS fluorescent lab
CsrRS is a two component regulator which is known to regulate 10-18 % of the genome of the closely related Streptococcus pyogenes. We aimed to look for transcriptional differences between pairs of nat
Our previous work has indicated that extracellular arginine concentrations may be an important stimulus for gene regulation in S gordonii. Here cells were cultured in a chemically defined medium (CDM