Transcriptional changes in Wnt antagonism- and TGFβ agonism-induced MSC chondrogenesis.

Wnt/β-catenin signaling is important in skeletal related-tissue development. We have found that Wnt/β-catenin antagonist XAV could replace traditionally used TGFβ3 as chondrogenic inducer. Here we applied gene expression array to characterize transcriptomic changes between XAV- and TGFβ-induced chondrogenic differentiation in human bone marrow (BM) and induced pluripotent stem cell (iPSC) derived MSCs. RNA samples were collected from human MSC pellets (1 BMMSC and 2 iPSC-MSC) with XAV- or TGFβ-based chondrogenic induction for 3 days.