AntibioINV

The manuscript presents very interesting results on the spread of bacterial resistance in relation to human activities. To achieve this, the authors sampled three rivers in regions with apparently no, medium, and high human impact. The results clearly demonstrate the link between human impact, even when modest population is observed, and the dissemination of bacterial resistance. The presence of bacterial resistance genes was also detected in regions with apparently no human impact. The authors based their conclusions on the detection of bacterial resistance genes and mobile elements using HT-PCR and bacterial cultures, as well as a 16S metabarcoding assay.