The role of Hippo pathway in ER positive breast cancer

We report the transcriptional difference of LATS1/2 gene knock out T47D cells with wild type control using RNA-seq technology. We further generated the chromatin binding of multiple proteins, including YAP, TEAD2, Estrogen receptor alpha, VGLL3 and several histone marks, as well as ATAC profiling between LATS1/2 gene knock out MCF7 cells with wild type control. Overall design: mRNA profiling between WT and LATS1/2 dKO T47D cell. Examination of multiple histone modifications, PolII, pPolII-S2, CTCF, YAP, TEAD2, ER-alpha, VGLL3 and ATAC chromatin signal between WT and LATS1/2 dKO MCF7 cells