RNA sequencing of spheroids from HUVECs for differences in Cis- and Trans-signaling of IL-11

Cytokines of the IL-6 family, such as IL-11, may influence angiogenesis. Our experiments showed opposite angiogenic effects on HUVECs depending on cis- or transsignaling of IL-11. Spheroids from the 3D angiogenesis model (spheroid sprouting assay) were lysed in QIAzole buffer by vigorous vortexing. The transcriptomic changes were analyzed by RNA sequencing. Spheroids from the spheroid sprouting assays were lysed in QIAzole buffer. A total of 12 samples from three independent spheroid assays were analyzed under the following conditions: VEGF, IL-11+VEGF, sIL-11R+VEGF, IL-11+sR+VEGF