In vitro development of embryos.

Zygotes were isolated at multiple sessions over a period of one year and in vitro development was studied. The numbers of embryos from all time points were cumulated. Wildtype littermates of α7 homozygous mutant mice were used as controls. The percentages of two cell stage, four cell stage, and blastocysts are calculated relative to the number of zygotes, two- and 4-cells stages, respectively.*p