mRNA-seq in VDR knockout and control THP-1 cells
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https://www.ncbi.nlm.nih.gov/sra/SRP280139
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资源简介：
The vitamin D receptor (VDR) has been knocked out in monocytic THP-1 cells after stimulation of ko and control cells with the the natural VDR ligand 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), quadruplicate mRNA-seq has been performed Overall design: The knockout has been performed by lentiviral CRISPR/Cas9 gene editing using a guideRNA (gRNA) directed against the VDR gene. The control cells have been created using a non-targeting gRNA (NTC). Prior to MRNA-seq, the cells were treated for 24 h with 100 nM 1,25(OH)2D3 or the solvent control (EtOH).
创建时间：
2021-12-07
