Transcript Level Expression Profiling: anticancer pathways of Smilax Glabra rhizome extract (SW) [HepG2 cells]. Homo sapiens

To find the possible signaling pathways which contribute to the anticancer effect of SW-treated HepG2 cells Global gene expression profiling is an ideal technique to obtain useful clues for exploration of the anticancer mechanism of SW. Through comparing microarray results between solvent- and SW-treated cells, differentially expressed genes were obtained (>1.5 fold). The microarray results were validated using real-time RT-PCR. We used the KEGG database, STRING database and GO database for further ananlysis, and therefore got the possible signaling pathways underlying the anticancer effect of SW. Overall design: We analyzed total RNA samples of solvent- and SW-treated HepG2 cells using the Affymetrix Human Gene 1.0 ST platform. No techinical replicates were performed.