Parameters of S. epidermidis biofilms in presence of heat-treated MBP-QQ proteins.

Coverslips coated with heat-treated (30 min at 65°C) and non-treated proteins MBP-QQ-5, MBP-QQ-7and MBP as control were used in flow chambers for S. epidermidis biofilm formation (3 x 108 cells/ mL). After 1 h adhesion, flow chambers were incubated for 20 h with a flow rate of 20 mL/h using 3% TSB medium. Biofilm characteristics thickness and volume were quantified using IMARIS software. Means and corresponding standard deviations of 2 independent biological replicates, each with 3 technical replicates are indicated.