SYSTEM AND METHOD FOR GENOME EDITING
收藏国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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Provided is a genome editing system for site-directed modification of a target sequence in the genome of a cell， which comprises a Cas9 nuclease variant having higher specificity as compared with the wild-type Cas9 nuclease or its expression construct， and an expression construct comprising the coding sequence of tRNA-gRNA fusion or ribozyme-gRNA fusion， or comprises an expression construct comprising the coding sequences of the Cas9 nuclease variant and tRNA-gRNA or ribozyme-gRNA both. Also provided is a method for genetically modifying a cell by introducing the genome editing system， which has high efficiency and high specificity. Provided is a genome editing system for site-directed modification of a target sequence in the genome of a cell， which comprises a Cas9 nuclease variant having higher specificity as compared with the wild-type Cas9 nuclease or its expression construct， and an expression construct comprising the coding sequence of tRNA-gRNA fusion or ribozyme-gRNA fusion， or comprises an expression construct comprising the coding sequences of the Cas9 nuclease variant and tRNA-gRNA or ribozyme-gRNA both. Also provided is a method for genetically modifying a cell by introducing the genome editing system， which has high efficiency and high specificity.
提供机构：
国家林业和草原科学数据中心
创建时间：
2023-02-12
