RNAseq data relating to manuscript: Prior Fc Receptor activation primes macrophages for increased sensitivity to IgG via long term and short term mechanisms.

Macrophages measure the ‘eat-me’ signal IgG to identify targets for
phagocytosis. We tested if prior encounters with IgG influence mouse
bone-marrow-derived macrophage appetite. IgG is recognized by the Fc
Receptor. To temporally control Fc Receptor activation, we engineered an
Fc Receptor that is activated by light-induced oligomerization of Cry2,
triggering phagocytosis. Using this tool, we demonstrate that subthreshold
Fc Receptor activation primes macrophages to be more sensitive to IgG in
future encounters. Macrophages that have previously experienced
subthreshold Fc Receptor activation eat more IgG-bound cancer cells.
Increased phagocytosis occurs by two discrete mechanisms – a short- and
long-term priming. Long-term priming requires new protein synthesis and
Erk activity. These long-term transcriptional changes show an increase in
immune response genes and a reprogramming of macrophages towards an M2
polarization. Short-term priming does not require new protein synthesis
and correlates with an increase in Fc Receptor mobility. Our work
demonstrates that IgG primes macrophages for increased phagocytosis,
suggesting that therapeutic antibodies may become more effective after
initial priming doses. This dataset relates to the included RNAseq data in
the Developmental Cell manuscript entitled: Prior Fc Receptor activation
primes macrophages for increased sensitivity to IgG via long term and
short term mechanisms.