PKR-dependent up-regulated genes upon HCV infection.

The Huh7.25.CD81 cells, seeded at 3.106 cells in 10-cm plates, were transfected after 24 hrs with 25 nM of siRNA Control or siRNA PKR using Fugene HD. 24 hrs post transfection, they were either mock-infected or infected for 2 hrs at 37°C with JFH1 (moi = 0.2) (three independent plates/sample). The medium was then removed and cells were incubated with complete DMEM for 12 hrs at 37°C. The cells were washed twice with TBS containing phosphatase and protease inhibitors, harvested by scraping, the cell pellets were centrifuged, the supernatants were removed and the pellets were frozen and stored at −80°C before being processed for micro-array.The list shows genes that were affected no more than twice by the depletion of PKR in the control cells (0.5