Whole genome methylation profiling.

anumber of differentially methylated probes within genebmethylated (M) and unmethylated (U) signal intensities and overall methylation levels (β) were calculated as the ratio of methylated to total signal (i.e. β = M / (M + U)) where β ranges from 0 (unmethylated) to 1 (methylated). Δβ was calculated for the difference in overall methylation levels between high and low SCDA level individuals.cnominal p-value, unadjusted for multiple comparisons; adjusted for estimated cell proportions.dnominal p-value, unadjusted for multiple comparisons; adjusted for estimated cell proportions, age, race and sex.Genes showing highest degree of differential methylation between individuals with high and low SCDA levels with |Δβ|>0.10 in the discovery and validation datasets.