Estimation of active site cysteines pKa values in wild type and mutant BC3987.
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The pKa values of the active site cysteines in BC3987 were estimated by pH titration resulting in pKa values 5.1 and 7.2 for the wild type and 7.2 for the T8A mutant. A cuvette containing 25 µM reduced protein in 1 mM polybuffer pH 9.3 was added 2 uL aliquots of 25 mM HCl and the change in thiolate absorption at 240 nm were followed with a UV-vis spectrometer.
创建时间：
2016-02-24
