KDM6A Regulates Immunogenicity of Multiple Myeloma (ChIP-seq)

We created isogenic mutiple myeloma and mouse embryonic fibroblast cell lines disrupted for KDM6A using CRISPR editing and Cre-mediated deletion. To detect KDM6A we inserted a dual HA tag sequence into the C-terminal of KDM6A by CRISPR-mediated editing. We also created one isogenic cell line harboring a jmjD-dead KDM6A where 2 amino acids essential for KDM6A enzymatic activity, H1146 and E1148 , were replaced with alanine residues Overall design: Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for KDM6A, HA epitote and the histone modifications H3K27me3, H3K27ac, H3K4me1 in KDM6A knock out and JmjD-dead cell lines.