Overexpression of ZmARF25 in Arabidopsis.

(A) The genomic PCR results for four homozygous T3 transgenic plants (35S::ZmARF25 #1–4) using Bar gene specific primer. PCR products were determined visually by running on 1% agarose gel and stained by Ethidium bromide. Col represents wild-type and #1–4 represent 35S::ZmARF25 transgenic lines, which is the same in Figure 3B; “+” represents the recombinant plasmid. (B) Relative expression levels of ZmARF25 gene in four homozygous T3 transgenic lines. Total RNA was isolated from the 30-day-old aerial of Arabidopsis. Data are represented as the mean ± SE (n = 3). ACTIN2 was used as an endogenous control.