Supplementary data: Canine Ticks, Tick-Borne Pathogens and Associated Risk Factors in Nigeria

Supplementary file 1 and 2 supports the manuscript titled "Canine Ticks, Tick-Borne Pathogens and Associated Risk Factors in Nigeria."

Supplementary file 1: contains additional figures and tables from laboratory and field investigations carried out in Nigeria and the UK and included the following:

Gel electrophoresis image showing PCR results for tick 16S rRNA gene amplification.

Summary tables of tick species identified morphologically and by PCR, with GenBank accession numbers.

Sample size calculations for estimating pathogen prevalence.

A flowchart outlining sample collection, processing, and analysis steps.

PCR protocols and primer details used for detecting tick-borne pathogens.

Univariate analysis of risk factors associated with tick infestation and pathogen prevalence based on POC and qPCR tests

Supplementary file 2: is a client consent and dog sample Collection FormThis form was used to record canine identification, clinical history, vaccination status, body condition, tick infestation level, tick species, and diagnostic findings  during sample collection. It also captures details on previous treatments, tentative diagnosis, and parasite detection.