transcriptomic analysis of A. fumigatus without or with ETD151 (0.156 µM) or amphotercin B (1.1µM)

The aim was to evaluate the transcriptomic pathway impacted by an ETD151 or amphotericine B treatment at 0, 30 minutes, 1 hour, 6 hours. 10^6 conidies of A. fumigatus were incubated in 10 ml YPD for 15 hours. ETD151 (0.156µM) or amphotericin B (1.1µM) was added in the media. After 0, 30 min, 1 hour or 6 hours, RNA from the fungal balls were then extracted using mechanical lysis followed by Nucleospin RNA kit (Macherey Nagel). RNA seq was performed using Illumina NextSeq 500 sequencer.