Microscopy data (3/3): interaction of the gene foxd5 with RNA polymerase II clusters in inhibitor-treated zebrafish embryos

Microscopy image data containing fluorescently labeled gene loci, recruited RNA polymerase II, and elongating RNA polymerase II. This data set is for the gene <em>foxd5</em> and is obtained from fixed zebrafish embryos, collected at the sphere stage of development. Embryos were treated prior to collection with control media, flavopiridol (10 µM, 30 minutes), JQ-1 (10 µM, 30 minutes), or hexanediol (3% weight/volume, 5 minutes). Data were recorded using an instant-SIM microscope (iSIM, VisiTech UK) with a 100X TIRF oil immersion objective (Nikon, NA 1.49, CFI SR HP Apo TIRF 100XAC Oil). Two samples were prepared per inhibitor condition, and images were recorded from 3-4 embryos per sample, as indicated in the file names. The image data are in the ND2 format (Nikon proprietary) and can be imported using the BioFormats importer (Open Microscopy Environment).