Data and code from: Departures from Mendelian inheritance of EPSPS gene copies in a herbicide-resistant weed

Here we report on inheritance and intra-individual variation in gene copy number associated with rapid evolution of herbicide resistance in the dioecious, agricultural weed Amaranthus palmeri. Copy number variation for glyphosate resistance gene EPSPS was quantified using digital droplet PCR within individuals, and for parents and offspring of controlled crosses with copy number ranging from 1 to ~160. The non-linear pattern of gene copy number inheritance from parent to offspring exhibits shifting directionality. Below the threshold of ~33 copies, the slope of the parent-offspring relation is high (1.60) because parents with fewer gene copies yield similar or increased mean offspring copy numbers, relative to parents. In contrast, above the threshold, the slope is low (0.21), and offspring of high copy number parents carry increasingly fewer EPSPS copies than parents. Intra-individual variance in copy number increases with mean individual copy number, likely contributing to variation i..., , # Data and code from: Departures from Mendelian inheritance of EPSPS gene copies in a herbicide-resistant weed

Dataset DOI: [10.5061/dryad.sbcc2frjv](https://doi.org/10.5061/dryad.sbcc2frjv)

## Description of the data and file structure

### Files and variables

#### **File: ‘inheritance_summary.csv’**

**Description:** n.b. this data file refers to offspring as progeny. Each row in this file summarizes an experimental cross between a female and male *Amaranthus palmeri*.  Crosses (family_ID) beginning with A, B, or C were part of the original set of experimental crosses (rows 2-31). Crosses beginning with ‘I’ were highly isolated crosses (rows 32-40).

**Variables**

·      family_ID = ID assigned to offspring / progeny generated by crossing parents specified by M_ID and F_ID

·      M_ID = paternal plant ID

·      F_ID = maternal plant ID

·      Maternal_CNV = EPSPS copy number of maternal plant

·      Paternal_CNV = EPSPS copy number of paternal plant

·      mean_parental_CNV =..., ,