Juveniles were dissociated in pools of 15 polyps. Polyps were continuously labeled with EdU (100uM, 2%DMSO) before dissociation, and fixed using Trypsin/formaldehyde. Cells were stained with 1μg/ml Fx
YB5 is a colon cancer cell line derived from the SW48 colon cancer cell line and contains a DNA methylated and silenced CMV promoter driving expression of a GFP reporter. The cells were treated with 1
