The role of CD146+ pericytes in skeletal muscle regeneration in vivo

This dataset supports a project investigating the role of the CD146+ cell population in skeletal muscle development and regeneration, as well as the impact of exogenous CD146+ cell engraftment on vascularization, fibrosis, and adipose tissue deposition in dystrophic skeletal muscle of mdx mice.CD146+ pericytes were isolated from hindlimb skeletal muscles of GFP-expressing mice and enriched using magnetic-activated cell sorting (MACS) based on CD146 surface expression. The cells were injected into skeletal muscles of mdx mice subjected to either BaCl₂-induced injury or treadmill-induced damage. The role of CD146⁺ cells during skeletal muscle development and regeneration was evaluated using histological staining and immunohistochemistry on tissue cryosections. RNA was extracted from either WT or mdx mouse skeletal muscles during regeneration, and qPCR was performed to monitor changes in gene expression levels.The impact of CD146+ cell engraftment was evaluated by quantifying fibers with centrally located nuclei (an indicator of regeneration), measuring muscle fiber cross-sectional area, and performing histological staining, immunohistochemistry, and qPCR analyses.This dataset includes:.xlsx (some with .ods copies) and .txt filesSpecifically:1.    CSA_BaCl2.xlsx - Excel file containing raw measurements of fiber size2.    2way_ANOVA_CSA_BaCl2_freaquency.txt - text file (.txt) showing statistical analysis (two-way ANOVA) of the fiber size distribution3.    central_nuclei_ BaCl2.xlsx - Excel file containing raw measurements of centrally nucleated fibers4.    CSA_treadmill.xlsx- Excel file containing raw measurements of fiber size5.    2way_ANOVA_CSA_treadmil_frequency.txt - text file (.txt) showing statistical analysis (two-way ANOVA) of the fiber size distribution6.    central_nuclei_ treadmill.xlsx- Excel file containing raw measurements of centrally nucleated fibers7.    Muscle_mass_BaCl.xlsx- Excel file containing raw measurements of muscle mass8.    Ordinary_one-way_ANOVA_of_muscle_mass_BaCl.txt - text file (.txt) showing statistical analysis (one-way ANOVA) of the muscle mass for BaCl injured muscles9.    Muscle_mass_treadmill.xlsx- Excel file containing raw measurements of muscle mass10. Ordinary_one-way_ANOVA_of_muscle_mass_treadmill.txt- text file (.txt) showing statistical analysis (one-way ANOVA) of the muscle mass for treadmill injured muscles11. Blood_vessels_area_BaCl_raw_data.xlsx- Excel file containing raw measurements of blood vessels area in BaCl injured muscles12. Ordinary_one-way_ANOVA_of_vessels_BaCl.txt - text file (.txt) showing statistical analysis (one-way ANOVA) of the blood vessels area for BaCl injured muscles13. Blood_vessels_area_treadmill_raw_data.xlsx - Excel file containing raw measurements of blood vessels area in treadmill injured muscles14. Ordinary_one-way_ANOVA_of_vessels_treadmill.txt - text file (.txt) showing statistical analysis (one-way ANOVA) of the blood vessels area for treadmill injured muscles15. Perilipin_raw_data.xlsx - Excel file containing raw measurements of perilipin expressing cells16. Ordinary_one-way_ANOVA_of_perilipin_BaCl.txt- text file (.txt) showing statistical analysis (one-way ANOVA) of the perilipin expressing cells in BaCl injured muscles17. Ordinary_one-way_ANOVA_of_perilipin_treadmill.txt - text file (.txt) showing statistical analysis (one-way ANOVA) of the perilipin expressing cells in treadmill injured muscles18. Masson_Trichrome_collagen_area_BaCl_raw_data.xlsx- Excel file containing raw measurements of collagen area in BaCl injured muscles19. Ordinary_one-way_ANOVA_of_collagen _area_AVE_BaCl.txt- text file (.txt) showing statistical analysis (one-way ANOVA) of of collagen area in in BaCl injured muscles20. Masson_Trichrome_collagen_area_treadmill_raw_data.xlsx- Excel file containing raw measurements of collagen area in treadmill injured muscles21. Unpaired_t_test_of_collagen_area_treadmill.txt - text file (.txt) showing statistical analysis (one-way ANOVA) of of collagen area in in treadmill injured muscles22. MCAM_WT_fluorescence_area_raw_data.xlsx - Excel file containing raw measurements of MCAM expressing cells area in WT muscles23. MCAM_WT_fluorescence_area_t_test.txt - text file (.txt) showing statistical analysis (t-test) of of collagen area in WT muscles24. MCAM_MDX_fluorescence_area.xlsx- Excel file containing raw measurements of MCAM expressing cells area in MDX muscles25. MCAM_MDX_fluorescence_area_ANOVA.txt - text file (.txt) showing statistical analysis (t-test) of of collagen area in MDX muscles26. qPCR_DMD_marker_expression_day_DMD_9_12.xlsx – Excel file containing raw Cq data for gene expression in MDX mouse muscles27. qPCR_DMD_marker_expression_day_DMD_5_8.xlsx - Excel file containing raw Cq data for gene expression in MDX mouse muscles28. qPCR_DMD_marker_expression_day_DMD_1_4.xlsx - Excel file containing raw Cq data for gene expression in MDX mouse muscles29. qPCR_WT_marker_expression_day_14.xlsx - Excel file containing raw Cq data for gene expression in WT mouse muscles30. qPCR_WT_marker_expression_day_7.xlsx - Excel file containing raw Cq data for gene expression in WT mouse muscles31. qPCR_WT_marker_expression_day_3.xlsx - Excel file containing raw Cq data for gene expression in WTmouse muscles32. qPCR_WT_marker_expression_control.xlsx - Excel file containing raw Cq data for gene expression in WT mouse muscles33. treadmill_part1_Abs Quant.txt - text file (.txt) containing raw Cq data for gene expression in treadmill injured mdx mouse muscles34. treadmill_part2_Abs Quant.txt - text file (.txt) containing raw Cq data for gene expression in treadmill injured mdx mouse muscles35. treadmill_part3_Abs Quant.txt - text file (.txt) containing raw Cq data for gene expression in treadmill injured mdx mouse muscles36. treadmill_part4_Abs Quant.txt - text file (.txt) containing raw Cq data for gene expression in treadmill injured mdx mouse muscles37. treadmill_part5_Abs Quant.txt - text file (.txt) containing raw Cq data for gene expression in treadmill injured mdx mouse muscles38. treadmill_part6_Abs Quant.txt - text file (.txt) containing raw Cq data for gene expression in treadmill injured mdx mouse muscles39. BaCl2_part1_Abs Quant.txt - text file (.txt) containing raw Cq data for gene expression in BaCl injured mdx mouse muscles40. BaCl2_part2_Abs Quant.txt - text file (.txt) containing raw Cq data for gene expression in BaCl injured mdx mouse muscles41. BaCl2_part3_Abs Quant.txt - text file (.txt) containing raw Cq data for gene expression in BaCl injured mdx mouse muscles42. BaCl2_part4_Abs Quant.txt - text file (.txt) containing raw Cq data for gene expression in BaCl injured mdx mouse muscles43. BaCl2_part5_Abs Quant.txt - text file (.txt) containing raw Cq data for gene expression in BaCl injured mdx mouse muscles