Transcriptome profiling of cybrids cells with and without m.14487T>C mutation

Transcriptome profiling is a sensitive strategy to uncover the change of mitochondrial to nucleus signaling due to the minor alternation of mitochondrial OXPHOS function. Therefore, we hypothesized that even a minor alternation of OXPHOS function can also remold transcriptome profiles in m.14487T>C mutant cybrid when compared with control cybrids. Our data suggest that a comparable mitochondrial OXPHOS function between m.14487T and m.14487C cybrids is not because of compensatory boost of mitochondrial biogenesis in m.14487C cybrids. Overall design: Examination of cybrids cells with and without m.14487T>C mutation from three different patients by deep sequencing,in triplicate,using Illumina Hiseq 2000.