Figure 5b-g: Representative Data showing the selection of an optimal RADAR sensor.

b-c) Six different organized guide RNA (ogRNA) sensors and linear sensors were cloned into the luciferase RADARS vector and transfected along with exogenous ADAR1p150 overexpression vector into HEK293FT cells. Target RNA ENO1 was modulated with either siRNA (b) or overexpression vector (c) as indicated. Cell culture supernatants were harvested 48 hours after transfection and analyzed for luciferase activity. In excel document, tab 'Gluc_div_Cluc_KD_exoADAR' is data for Figure 5b. Tab 'Gluc_div_Gluc_ovexp_exoADAR' is data for Figure 5c.

d-e) The same guides and target modulation conditions as in (b-c) with luciferase readout, but leveraging endogenous ADAR expression (no exogenous ADAR1p150 added in transfection conditions).
In excel document, tab 'Gluc_div_Cluc_KD_endoADAR' is data for Figure 5d. Tab 'Gluc_div_Gluc_ovexp_endoADAR' is data for Figure 5e.

f-g) The same organized ogRNA guides and linear guides as in (b-e) were cloned into a RADARS vector with mNeon cargo and transfected into HEK293FT cells supplemented with exogenous ADAR1p150 overexpression vector. Target RNA ENO1 was modulated with either siRNA (f) or overexpression vector (g) as indicated.
In excel document, tab '%GFP_flow_KD_exoADAR' is data for Figure 5f. Tab '%GFP_flow_ovexp_exoADAR' is data for Figure 5g.