Results obtained with the inhibitors which passed the initial screening.

aPercentages indicate the average percent increase in mean lifespan obtained in the number of independent trials indicated in parentheses. Because maximum lifespan (lifespan of the longest lived 10%) was variable due to the presence of occasional long-lived outliers, this metric was not used.
bNC indicates that the effects of the drugs on food consumption were not statistically different than control in FPAs and/or CAFE assays (see Materials and Methods for the details of the assays and statistical tests used). These FPAs used 6 control and 6 treated 8 ounce fly bottles containing 30 to 50 flies each. The absence of a notation indicates that the test was not performed for that drug. Where a range of optimum concentrations of the drug is given, the assay was performed at the highest concentration in the range.
cH8 was rapamycin (sirolimus) in the Biomol library. Because of its poor bioavailability, we tested everolimus, its more bioavailable derivative.