Single-cell dynamic RNA and glycosylation sequencing reveal the process of iPSC differentiation into HSCs in vitro

Understanding the differentiation process of human induced pluripotent stem cells (iPSCs) into hematopoietic stem/progenitor cells (HSPCs) is essential for advancing regenerative medicine and gaining insights into embryonic hematopoiesis. However, the connection between transcriptomic dynamics and glycation levels during this process remains unclear. In this study, we conducted a systematic analysis of the dynamic transcriptome and glycation changes of HSPCs derived from iPSCs during in vitro differentiation. Comprehensive analysis showed that there is a significant correlation between staged hematopoietic differentiation of iPSC-derived HSPCs and the degree of glycation, particularly during the endothelial differentiation stage. Additionally, there are similarities between in vivo embryonic hematopoiesis and in vitro differentiation models, which reinforces the utility of these models for studying human hematopoiesis. This study provides important insights into the link between the transcriptome and glycation of HSPCs derived from iPSCs. Cell samples from D0, D2, D4, D6, D8, and D10 in the in vitro model of iPSC-derived HSPC were collected and analyzed using single-cell RNA sequencing (scRNA-seq), single-cell Dynamic RNA-seq (DynaSCOPE), and single-cell Glycosylation-seq (ProMoSCOPE).