Early transcriptomic responses of rice leaves to herbivory by Spodoptera frugiperda

In this experiment we aimed to decipher the transcription response of rice to the different cues of an herbivory attack from the chewing caterpillar Spodoptera frugiperda. We performed a herbivory mimic procedure on seedlings and applied mechanical wounding (WOS), application of herbivore oral secretions (OS) after wounding (WOS+) and dysbiotic OS (WOS-) on rice leaves. RNA was extracted from control and treated leaves after two hours. 5 biological replicates corresponding to 5 pooled leaves were performed for each of the four condition