Regulatory activity of the wild-type AraR protein and mutants E30 and Y5 over an araA-lacZ promoter fusion (wild-type and mutated variants).

aβ-Galactosidase activities of the B. subtilis strains grown in the absence (−ara) or presence (+ara) of arabinose. Values represent the average and standard deviation of at least three independent experiments, each assayed in duplicate. MU Miller units.bR.I. (Repression Index) indicates the regulatory activity, calculated as the ratio between values obtained in the presence and in the absence of inducer.Regulatory activity of the wild-type AraR protein and mutants E30 and Y5 over an araA-lacZ promoter fusion (wild-type and mutated variants).