DNA Methylation Potential Energy Landscape Analysis of Pediatric Pre-B cell Acute Lymphoblastic Leukemia (ALL) and Normal B cells and precursors

Acute lymphoblastic leukemia (ALL) is the most common cancer in children. We applied WGBS and the informME analysis pipeline to investigate the role of DNA methylation stochasticity in pre-B cell ALL. Overall design: WGBS was carried out on pediatric pre-B cell acute lymphoblastic leukemia primary samples, cell lines, and normal umbilical cord blood CD19+ cells. The informME analytic pipeline was applied to these data as well as to existing WGBS data for normal B cell precursors. Single-cell RNA-seq of ETV6-RUNX1 pre-B acute lymphoblastic leukemia primary patient samples and normal umbilical cord blood derived CD19+ cells. RNA-seq of ETV6-RUNX1 pre-B acute lymphoblastic leukemia primary patient samples and normal umbilical cord blood derived CD19+ cells. Raw data for the PreB2-1 and -2 samples is available elsewhere (EGA download, previously released data).