Comparative mass-spectrometry of pTyr immunoprecipitated zebrafish lysates.

Zebrafish embryos were injected at the 1-cell stage with synthetic mRNA constructs encoding WT Shp2, NS (D61G) Shp2 or LS (A462T) Shp2 and co-injected with mRNA encoding eGFP. Lysates were subjected to mass spectrometry as described in Materials and Methods. Normalized ratios (Log2 scale) based on total levels of non-phosphorylated peptides are given. *Protein name based on BLAST sequence of peptide. Accession numbers from BLAST hits are used for non-annotated peptides. **pRS score <75, phosphorylation site could not be determined but the most commonly identified site from Phsophosite.org is used. a-n: indicators for Figure 1C. Comparative mass-spectrometry of pTyr immunoprecipitated zebrafish lysates.