Comparison of RNA sequencing profiles in domed and flat colonies of ocular surface epithelial stem cells induced from human pluripotent stem cell

RNA-seq analysis revealed that genes associated with lacrimal gland development, such as BARX2, SIX1, SIX2, SOX9, and KRT15, were upregulated in domed colonies compared to flat colonies. In flat colonies, mucosal epithelial keratin, KRT13 and the corneal epithelial progenitor cell markers, CDH2 and NGFR were up-regulated, along with YAP1 and its downstream genes, CTGF and CYR61, which are involved in epithelial development. RNA sequencing profiles of domed and flat colonies of ocular surface epithelial stem cells