Tsukamurella tyrosinosolvens genome from Metagenome of Pleural fluid grown in Mycobacteria Growth Indicator Tube

A complete/near-complete genome of Tsukamurella tyrosinosolvans was extracted from shotgun metagenome sequencing of a pleural fluid grown in Mycobacteria Growth Indicator Tube. The sample was suspected for Mycobacterium tuberculosis. Tuberculous pleuritis is a common extrapulmonary manifestation often presented with nonspecific symptoms. Misidentification of Tsukamurella as Mycobacterium tuberculosis is a well-recognized diagnostic challenge because the two genera share several morphological, biochemical, and clinical features. This particular sample showed acid-fast filamentous, Gram-positive cells, and showed growth in Mycobacteria Growth Indicator Tube (MGIT) from which DNA was extracted for sequencing. This genome can be explored for further studies on pathogenicity of this species.