Chromatin accessibility changes in response to TGFB in human pancreatic stellate cells with knockdown of PIN1

Pancreatic stellate cells can be activated to a myofibroblast phenotype in response to TGFB treatment. The goal of this study was to determine how loss of PIN1 affects stellate cell state at baseline and in response to TGFB treatment. Primary human pancreatic stellate cells (PSCs) were cultured in Stellate Cell Media. For knockdown studies, PSCs were infected with a pLKO-shPIN1 lentivirus or an shSCR control lentivirus. Stable pools were generated with puromycin selection. These cells were plated, and then treated for 8 hours with 5 ng/mL TGFB1. Nuclei were isolated and used for single cell combinatorial indexing ATAC-Seq