Data from: BrAD-seq: Breath Adapter Directional sequencing: a streamlined, ultra-simple and fast library preparation protocol for strand specific mRNA library construction

Next Generation Sequencing (NGS) is driving rapid advancement in
biological understanding and RNA-sequencing (RNA-seq) has become an
indispensable tool for biology and medicine. There is a growing need for
access to these technologies although preparation of NGS libraries remains
a bottleneck to wider adoption. Here we report a novel method for the
production of strand specific RNA-seq libraries utilizing inherent
properties of double-stranded cDNA to capture and incorporate a sequencing
adapter. Breath Adapter Directional sequencing (BrAD-seq) reduces sample
handling and requires far fewer enzymatic steps than most available
methods to produce high quality strand-specific RNA-seq libraries. The
method we present is optimized for 3-prime Digital Gene Expression (DGE)
libraries and can easily extend to full transcript coverage shotgun (SHO)
type strand-specific libraries and is modularized to accommodate a
diversity of RNA and DNA input materials. BrAD-seq offers a highly
streamlined and inexpensive option for RNA-seq libraries.