Species discovery and delimitation in ground beetles of the subgenus Trepanedoris: matrices and trees

We infer species boundaries in small ground beetles of the genus
Bembidion, presenting the discoveries made by a class of students in an
experiential learning course at Oregon State University. Focusing on the
Bembidion connivens group of subgenus Trepanedorisoutside of California,
we integrate data from external structure and male genitalia, DNA
sequences of one mitochondrial gene and six nuclear genes, and geographic
distribution. We analyze the DNA data using Bayesian multispecies
coalescent methods to help infer reproductive communities or species;
SPEEDEMON tended to split species more than what our integrated approach
inferred, whereas DELINEATE tended to lump more than synthetic inference.
We infer 12 species in North America outside of California, including four
new species. The files in this archive include a Mesquite NEXUS file
containing DNA data matrices and inferred trees, Supplementary Tables
including localities of specimens and GenBank accession numbers of
analyzed DNA sequences, and Supplementary Figures showing intermediate
results in the DELINEATE analysis.