Data for: Defective kernel 66 encodes a GTPase essential for kernel development in maize

Mitochondria is a semi-autonomous organelle that provides energy for cell
activities through oxidative phosphorylation. In this study, we identified
a defective kernel 66 (dek66)-mutant maize with defective kernels. We
characterized the candidate gene DEK66, encoding a ribosomal
assembly factor, located in mitochondria, and possessing GTPase activity
(the GTPase belonged to ribosome biogenesis GTPase A family). In the dek66
mutant, abolishment of mitochondrial structure and function led to the
accumulation of reactive oxygen species and promoted programmed cell death
in endosperm cells. Furthermore, transcript levels of most of the key
genes associated with nutrient storage, mitochondrial respiratory chain
complex, and mitochondrial ribosomes in the dek66 mutant were
significantly altered. Collectively, the results suggested that DEK66 is
essential for the development of maize kernels via affecting mitochondrial
function. This study provided a reference to understand the impact of
mitochondrial ribosomal assembly factors in maize kernel development.