Experimental Data Set for Study of Groundwater Heavy Metal In-situ Fluorescence Detection Device

A fluorescence in-situ detection device for heavy metals in groundwater was developed with an optical probe using UV light-emitting diodes (LEDs) as the light source, a confocal optical path design, a fluorescence collection angle range of up to 102°, and an outer diameter of no more than 50 mm, achieving miniaturization and low cost. Comparative performance tests were conducted using a commercial benchtop fluorescence spectrophotometer as a reference device, equipped with the same divalent mercury ion (Hg2+) fluorescent probe.The data set includes1. Light source wavelength parameter test: UV LED (YL-UVALP-TO18-365, Yonglin Optoelectronics Co., Ltd., China) was selected as the light source with a nominal wavelength of 365 nm, a measured central wavelength of 371 nm, a luminescence angle of 15°, and a stable optical power of 20 mW. the direct LED output spectral curve measured by an Ocean Optics spectrometer (QE Pro, Ocean Optics, Inc., USA). The spectral profile of the output.2. The test results of the fluorescent probe used with different concentrations of divalent mercury ions on a commercial benchtop fluorescence spectrophotometer: Lumina-type fluorescence spectrophotometer from Thermo Scientific, USA, was used as the reference equipment. The excitation wavelength selected in this experiment was 360 nm. the fluorescent probe was placed in a standard quartz cuvette before detection, and then the Hg2+ concentration gradient titration experiment was carried out, and the titration concentration range was from 0 ppb to 1000 ppb.3. The absorption spectrum of the fluorescent probe used.4. Test results of the used fluorescent probe with different concentrations of divalent mercury ions on the self-research device: different concentrations of Hg2+ solutions were used to mix with the fluorescent probe and repeat the test in the liquid chamber. A pipette gun was used to take 10 mL of fluorescent probe with a concentration of 10-3 mol/L, and the corresponding concentration and volume of Hg2+ was mixed well, and the total solution volume was always controlled to be 1 mL, so that the Hg2+ concentration was from 0 ppb to 1000 ppb, and the fluorescence spectral changes were measured on the device with a spectral integration time of 100 ms.