16S rRNA gene-based sequencing, microbial diversity with long-life various foods.

The aim of this study was to assess whether the bacteria-specific primer pair 335f/769r, which was developed to amplify bacterial DNA in plant DNA-rich samples, is suitable for use in metagenomic analyses of complex food matrices that contain plant DNA. These results should aid in validating these primer pairs with methodologies for performing food microbial population analysis. There is substantial loss of information due to chloroplast and mitochondrial sequencing reads, which are useless for such analyses, from complex matrix environmental samples by high-throughput sequencing.