Host immune response in brains of mouse model of cryptococcal post-infectious inflammatory response syndrome (cPIIRS), cPIIRS mice treated with Ruxolitinib, and naïve control mice

Transcriptional profiling of host immune response in brains of mouse model of cryptococcal post-infectious inflammatory response syndrome (cPIIRS), cPIIRS mice treated with Ruxolitinib, and naïve control mice using the NanoString nCounter Mouse Neuroinflammation Panel v1.0. NanoString nCounter assay was performed to measure the expression of neuroinflammatory genes in the brains of cPIIRS mice (C57BL/6Tac, 21 day post-infection) compared to cPIIRS mice treated with Ruxolitinib, and naïve mice. In this study, RNA was isolated from whole brains of cPIIRS mice (female C57BL/6Tac, 21 day post-infection), cPIIRS mice treated with Ruxolitinib (female C57BL/6Tac, 21 day post-infection, 1 g/kg in Nutragel diet per day), and naïve mice (female C57BL/6Tac) and the expression of 757 genes was measured.