Transcriptomic Profiling of siCHRNA5 and siTP53 Perturbations in ER-Positive MCF7 Breast Cancer Cells

Estrogen receptor (ER)-positive MCF7 breast cancer cells were treated with siRNAs targeting CHRNA5, TP53, or both in combination to investigate TP53-dependent and independent transcriptional responses to CHRNA5 knockdown. Previous studies have shown that CHRNA5 silencing induces cell death via TP53 pathway activation. To further elucidate the molecular mechanisms underlying this effect, we performed RNA sequencing following 72-hour treatments with siCHRNA5, siTP53, and their combination, alongside appropriate siRNA controls. This dataset provides a valuable resource for understanding the role of CHRNA5 in ER-positive breast cancer and its interaction with TP53 signaling, with implications for therapeutic targeting strategies. Overall design: MCF7 cells were treated with siRNAs targeting CHRNA5, TP53, or both, alongside controls, to investigate TP53-dependent and independent transcriptional responses to CHRNA5 silencing in ER-positive breast cancer using RNA sequencing.