Pulsed SILAC quantitative proteomics profiling to understand the protective role of ginsentide TP1 against hypoxia.

This project used a Pulsed SILAC (pSILAC) proteomics approach to pulsed/chase the incorporation of stable isotope-labeled lysine (13C6 and 15N2 L-lysine) and arginine (13C6 L-arginine) into newly synthesized proteins and profiled de novo protein synthesis in endothelial cells (HUVEC-CS) subjected to hypoxia stress with or without ginsentideTP1.