Transcription profiling by array of mouse sciatic nerve endoneurium and dorsal root ganglions isolated from pre-symptomatic and early symptomatic diabetes mellitus type 1 (DM1) model animals

Even though the pathological changes, including decreased MNCV and progressive neuroanatomical alterations affecting neuronal structures are relatively well described, molecular mechanisms underlying DPN remain poorly understood. To analyze more extensively the molecular changes leading to the development of the DPN, we performed a gene expression profiling of the sciatic nerve endoneurium and DRGs isolated from pre-symptomatic and early symptomatic DM1 mouse model. These experiments should allow us to determine to which extent the two compartments of the nerve (the DRG composed mostly of neurons and the endoneurium containing mostly Schwann cells) are affected by diabetes, to analyze the possibility of a chronological order in alterations observed in these compartments and to correlate phenotypic changes observed in AkitaIns2/+ mice (a model of diabetes type I) with underlying transcriptional alterations.