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single cell RNA-sequencing of diabetic mouse kidney

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single-cell RNA sequencing of renal cells from type 2 diabetes mice (BTBR ob/ob) at the early stage of DN. Glomeruli were enriched by depleting the PT segment from the kidneys using Percoll density gradient centrifugation, then the single-cell RNA sequencing was performed to BTBR ob/ob vs. BTBR +/+ in glomerular cells.

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